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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Acanthaster Crown of Thorns Seastar COI 126 TCCGACTACCCGGACGCCTATAC AGTGGTTCGCTGGGAAGTGAAGG CTATCTCATCCATAGGCAGCAC 99
Acheilognathus CYTB N/A GCGTTGTTCTCCCCCAATTT TAAAATGGCGTAGGCGAACA TCCCCCCCACATCAAACCCGAGT N/A
Acipenser Siberian sturgeon CR 98 GACAGTAATTGTAGAGTTTC CAGTAACAGGCTGATTATG N/A N/A
Acipenser Lake sturgeon COI 280 CGGCACAGCCCTCAGCCTTC GGCTCCGGCCTCTACCCCAG N/A N/A
Acipenser Lake sturgeon COI 140 GGGGCCCCAGACATGGCATT CCCGCCAGCGGAGGGTAAAC N/A N/A
Acipenser Lake sturgeon COI 126 CTGGGGTAGAGGCCGGAGCC TGGACGAAACCCCAGCCAGGT N/A N/A
Acipenser Lake sturgeon COI 242 CTCCCTTCACCTGGCTGGGGT TGGGGTCTCCTCCTCCGGCT N/A N/A
Acipenser lake sturgeon COI 57 ACCCGGTGCCCTGCTT GGCGTGGGCTGTAACGATA CGATGATCAGATCTAC N/A
Acipenser lake sturgeon CYTB 105 ATGAATCGGAGGCCAACCAG TAAGCCTGTCAGAGGGAGGG CCTCCACAGTTTATTTTGCCCTATTCCT N/A
Acipenser North American Green Sturgeon COI <150 AGGGAAAAAATGGTTAGGTCTACAGA CCCCACTGGCGGGAAA CTCCCGCATGGGCTA 94
Acipenser Green Sturgeon COI N/A AGGGAAAAAATGGTTAGGTCTACAGA CCCCACTGGCGGGAAA CTCCCGCATGGGCTA N/A
Acipenser Gulf sturgeon CYTB (∼150 bp) CGAGCGCTAGCTTAAAACCCA CCCATAGCCGGCTTCAAAAGA N/A N/A
Acipenser White Sturgeon CYTB <150 CCCCGTTTGCATGAATGTTT CGCCCACATCTGCCGAGAT ATTAGTCATCCGTAATTCA 101
Acris Blanchard’s cricket frog COI 102 GGACGGGCTGGACAGTATACC AAGAAACCCCTGCGA GATGGTATACC AGCACATGCTGGCCCATATACC 97.49
Aedes Tiger mosquito ITS-1 35+17+19 GTCAGCAGGGCCGAACC GACGACCCGCCACTTAGCT CAGGGCACATACGTCCGCTTTGGTT 96.9
Aedes Asian bush mosquito COI 77+FP+RP GCTCCAGATATAGCTTTCCCTCG GGATAAACAGTTCATCCAGTTCCAG ACCTTACTACTTTCAAGTAGAATG 93.5
Aedes COI 77 CCCAGATATAGCCTTCCCCCG GGATAAACAGTTCAT CCTGTCCCAG CTCCCTCATTAACTCTACTACTTTCAAGAAGTATAGTAG 97.5
Alasmidonta dwarf wedgemussel COI 277 CACACCCCTTTCAACCAACG GTTGGCTTTAAGACTTTTAATC TGGTCATCTCCTAACAACCTTCCAGG 96.5
Alburnoides CYTB 63 GGGCGCCACCGTCAT TGAACAAGTATGTCTCCCATGTAAGG CGA ATC TCC TTT CAG CAG T 93
Alosa River herring COI ≈50 ATGAGCTTCTGACTACTT GATAGTTAGATCGACGGA CGCGATCGGATGAACAGTCTACCCGCCCTTGATCGCG N/A