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Genus Species Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Acanthopagrus schlegelii Blackhead seabream Cytb 129 CTGTCTGCCGTCCCCTACA TATGGCGGCTACGATAAAAGGA FAM–TCAGTTGACAACGCAACCCTAACCCG–TAMRA 0.79–0.87
Acipenser ruthenus Sterlet 16S – 5′–TCTACCGTCACCCAGGTCAT–3′ 5′–CGCCTGTTAAGGTTGTGTTCTTTT–3′ 5′–FAM–GAGAGGTACAGCTCTCTTG–MGB–Q500–3′ 99.6
Acipenser fulvescens Lake sturgeon COI 57 ACCCGGTGCCCTGCTT GGCGTGGGCTGTAACGATA FAM–CGATGATCAGATCTAC–MGBNFQ –
Acipenser fulvescens Lake sturgeon Cytb 105 ATGAATCGGAGGCCAACCAG TAAGCCTGTCAGAGGGAGGG VIC–CCTCCACAGTTTATTTTGCCCTATTCCT–MGBNFQ –
Alasmidonta heterodon Dwarf wedgemussel COI 277 CACACCCCTTTCAACCAACG GTTGGCTTTAAGACTTTTAATC TGGTCATCTCCTAACAACCTTCCAGG 0.965
Alburnoides bipunctatus Cytb 63 5`–GGGCGCCACCGTCAT–3′ 5′–TGAACAAGTATGTCTCCCATGTAAGG–3′ 6FAM–CGA ATC TCC TTT CAG CAG T–MGBNFQ 93
Ammocrypta pellucida Eastern sand darter COI 83 5′‐CTTGTTCGTGTGGGCTGTGTT‐3′ 5′‐GCAAGAACTGGGAGCGAAAG‐3′ 5′‐6FAM‐ATTACCGCTGTTCTTC‐3′ 92–113
Anguilla japonica Japanese eel 16S 154 AATCAGTAATAAGAGGGCCCAAGC TGTTGGGTTAACGGTTTGTGGTA CACATGTGTAAGTCAGAACGGACCGACC 82.4 to 88.9
Aphanomyces astaci ITS1 59 AAGGCTTGT GCTGGGATGTT CTTCTTGC GAAACCTTCTGCTA 6 – FAM – TTCGGGACGACCCMGBNFQ 99.33
Aphanomyces astaci ITS – 5′–AAGGCTTGTGCTGGGATGTT–3′ 5′–CTTCTTGCGAAACCTTCTGCTA–3′ 5′–6–FAM–TTCGGGACGACCC–MGBNFQ–3′ –
Aphanomyces astaci ITS 58 AAGGCTTGTGCTGGGATGTT CTTCTTGCGAAACCTTCTGCTA FAM‐TTCGGGACGACCC‐MGBNFQ –
Astacus astacus European noble crayfish COI 65 GATTAGAGGAATAGTAGAGAG CTGATGCTAAAGGGGGATAA FAM‐AGGAGTAGGGACAGGATGAACT‐BHQ1 –
Aurelia aurita Moon Jellyfish COI 120 TTACTACCCCCAGCTCTGCTTT TACTGAACCACCGGAATGG FAM–ATGAACAATTTATCCCCCCCTAAGCGCA–TAMRA 0.90–0.95
Austropotamobius pallipes White–clawed crayfish COI 96 5′–GGG TTA GTG GAG AGA GGG GT–3′ 5′–AAT CCC CAG ATC CAC AGA CG–3′ 5′–TCA GCT ATT GCC CAC GCA–3′ 95.22–98.04
Chelonia mydas Green Sea Turtle Control Region (D–Loop) 488 CGG TCC CCA AAA CCG GAA TCC TAT GCA AGT AAA ACT ACC GTA TGC CAG GTT – 85.8
Chelonia mydas Green Sea Turtle Control Region (D–Loop) 253 GGC CCA CAT AAC TGA TAC CTG CCG A GTC TCG GAT TTA GGG GTT TG – 105
Chrysaora pacifica Japanese sea nettle COI 231 CCCAGATATGGCTTTTCCTAGA TGAGTGAGCTTGTATAGCTGATA FAM–TAGGATCCTCCCTAATTG–NFQ–MGB 0.90–0.93
Clarias gariepinus North African Sharptooth Catfish COI 150 ACTCACAACCCAAATCGTTAAT 5′–CAGGGCAGGCAAGACCTCCT–3′ – 1
Clonophis kirtlandii Kirtland’s Snake COI 135 TCCCCTTGTTCGTTTGGTCA CACCTCCGCATGGATCGAA ACCGACCGAAACATTAACACCTCCTT 80
Coregonus sardinella least cisco COI or cytb? 129 CTCGCCCTCCTATTCTCTATCCTA GATGAGCATGTCTGCTACCAGG FAM‐CATTCTTCATACCTCTAAGCAG‐MGB‐NFQ –