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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Procambarus Louisianna crayFish COI 73 GGAGTTGGAACAGGATGGACT AATCTACAGATGCTCCCGCA CCTCCTTTAGCTTCTGCTATTGCTC N/A
Procambarus crayfish CYTB 109 CCTGTGGTAGAGGTTGGCAG CCGTAGCCCCAGAAGACAAT N/A N/A
Procambarus crayfish CYTB 96 CAGAAGCTAAAGGAGATAA AACTAGGGGTATAGTTGAGAG N/A N/A
Prosopium Round Whitefish COI 125 AATGTAATCGTCACGGCCCA CGGGGGAATGCTATATCGGG TGACTAATTCCCCTTATGATCGGAGCA 100
Prosopium Round whiteFish COI 125 AATGTAATCGTCACGGCCCA CGGGGGAATGCTATATCGGG TGACTAATTCCCCTTATGATCGGAGCA N/A
Proterorhinus Ponto-Caspian goby CYTB 125 CCTCTAACATTTCTGCC GACGAGAAGGCTGTGGC N/A N/A
Proterorhinus Ponto-Caspian goby CYTB 125 CCCTCTAACATTTCTGCC GACGAGAAGGCTGTGGC N/A N/A
Proteus olm 16S 157 TACTGCCTGCCCAGTGACAA TGCACGAGGAGATCAATTTCG TTACGCTACCTTTGCACG N/A
Proteus olm CR 106 GCGTTAATTACAAGGTGCACTTGG TGTACCAGGTATTACCTTTAATGTTGG N/A N/A
Proteus olm CR 153 CTGCCTGCCCAGTGACAACA CACGAGGAGATCAATTTCGCAGA N/A N/A
Proteus black olm CYTB 146 CATCCTACTGACATGGATCGGA GGCAGAGGTCTAGGAGTTTGTTTTC CATAATCCCATCAGCCGGA N/A
Proteus white olm CYTB 143 CAGATGCCATCGTACTGACCTG TAGGAGTTTGTTTTCAGCCCATC ATCGCCCTAATTCCATC N/A
Pseudacris Spring peeper COI 99 CCATCTTTCCTTCTTCTCCTCG TGCATGTGCTAAGTT CCCAGCCTCCTCG GTGTAGAGGCAGGTGCCTCCTCG 102.1
Pseudacris Boreal chorus frog COI 71 CTTGCTGGAAATTTAGCACACG ATAGCTCCTAGGATT GAAGACACACCGCACACG GGCCCATCAGTTGATGCACACG 94.9
Pseudacris Ornate chorus frog N/A 91 TRGGTGTCTGCCTCATTCTTCA GGCYACGGATGAGAAGGCTAT ATCGCCACTGGCCTATTT N/A
Pseudacris Ornate Chorus Frog N/A 91 TRGGTGTCTGCCTCATTCTTCA GGCYACGGATGAGAAGGCTAT Quasar670-ATCGCCACTGGCCTATTT-BHQPlus 80-120%
Pseudocaranx Striped jack CYTB 130 ACGCAGTTTTTATTCTGGC CTAGGAAGTATAGGGC CGTGGCTATCCTCACCTGAATCGGG 73%-98%
Pseudophryne southern corroboree frog ND4 155 GGAGTCAAATCATCCTCCCAC TAAAGCTGCTAAGACAAGTGAGA ACCCCCATCAACCAACTTCA 90
Pseudophryne northern corroboree frog ND4 155 GGAGTCAAATCATCCTCCCAC TAAAGCTGCTAAGACAAGTGAGA ACCCCCATCAACCAACTTCA 90
Pseudorasbora Topmouth gudgeon COI 350 CCTCTTCCGGAGTAGAGGCT TAGGATTGGGTCTCCTCCCC N/A N/A