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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Salvelinus Dolly Varden CYTB 145 CCGCCACAGTACTTCACCTTCTA AGGCCAAGCAATATAGCTACGAAA FAM-CCGACAAAATCTCATTCC-MGB-NFQ 99.9
Salvelinus Dolly Varden CYTB 139 CTTATTTGCCTACGCAATTCTCC GTGAGGATGAGTATGTCTGCTACCA TTGTCCCGATCCTCCACA N/A
Salvelinus Lake trout COI 66 GGGCCTCCGTTGATTTAACTATC TGGCCCCTAAAATTGAGGAA CTCTCTTCATTTAGCTGGC 86%-99%
Salvelinus lake trout COI 66 GGGCCTCCGTTGATTTAACTATC TGGCCCCTAAAATTGAGGAA CTCTCTTCATTTAGCTGGC 86-99
Sander sister taxa sauger CYTB 112 TGGGGTCATCCTCCTTCTRAT TGCAGATAAGAGGTTAGTAATGACGGTA FAM-TTTGTAGGGTATGTATTACCCTGA-MGBNFQ 95.78
Sander walleye CYTB 175 CTATTATACTATTTACCCTCGGGCTCG GTCGATTGAACAATGAAGTATTTTGC FAM-TAATTGCCTGAATGGGTC-MGBNFQ 97.67
Sardinops Pacific Sardine D-loop 107 TCAGAGCAAGGAAACTG CCGACGGTATACATAAAGC ATTTACCGGGTGCCCTTCATT 91
Scaphirhynchus Alabama sturgeon CYTB (∼150 bp) CTCCTAGGCCTCTGCCTTATT CTTTTTGGAGGTAGGAGCCGT N/A N/A
Scardinius Common rudd COI 270 CAGGTAATCTAGCCCACGCA GCTGGGTCGAAGAATGTGGT N/A N/A
Scardinius Common rudd COI 190 CCCACCACTCGCAGGTAATC AGCTGTTACCAGTACGGCTC N/A N/A
Scardinius Eurasian rudd D-loop 203 AAGAAACCACCAACCGGTCC CTCGGCATGTGGGGTAATGA N/A N/A
Schistosoma COI 162 CAGGGGTTTCAAGTCTAATTGGAT CAAATAATAACATCGTTATTCCTCTGG TTCAAATGTTCGATAATA N/A
Scomber Pacific Chub Mackerel COI 107 GCTGAACAGTTTATCCTCCCCTCG CCCAAGGATTGAGGAAACACCTGCTAG TGGGAACCTGGCACACGCCGGG 95
Scomber Pacific Chub Mackerel COI N/A GCTGAACAGTTTATCCTCCCCTCG TGGGAACCTGGCACACGCCGGG 92.7
Scomber Atlantic mackerel CYTB 100 TTCCCTGCTTGGTCTCTGTT GGCGACTGAGTTGAATGCTG TTCCCAAATCCTCACAGGACTATTC N/A
Shinisaurus crocodile lizard CYTB 97 GCCCACATCTGCCGAGAT CGATATGAAGGTAAATGCAGAAGAAA N/A 92.12
Shortnose COI 252 TGATCTAACCATTTTTTCCCTG TGTAATACCTGCGGCTAGGA N/A N/A
Silurus Wels catFish 16S 303 ATGAATGGTGGAACGAGGGC GCTGGTGGCCGGATCTTAG N/A N/A
Silurus Wels catFish 16S 219 CGTGCAGAAGCGGACATATT TCAGATGTTCTGTGGCTTAGAA N/A N/A
Silurus Wels catFish COI 239 GCAGGAACAGGATGAACCGT ATCGGCAGGGACAGGAGTAA N/A N/A