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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Trichechus West Indian manatee CYTB 69 CGCTAACCGCATTCTCTTCAG GGT AGC GAA TGA TYCAAC CAT AGT TTAACCGCATTCTCTTCAG (5’-CCC ACA TTT GCC GAGAC-3’)TAACCGCATTCTCTTCAG 90-110%
Trichechus Manatees CYTB 69 CGCTAACCGCATTCTCTTCAG GGTAGCGAATGATYCAACCATAGTT CCCACATTTGCCGAGAC 95.25
Triturus great crested newt CYTB 81 CGTAAACTACGGCTGACTAGTACGAA CCGATGTGTATGTAGATGCAAACA CATCCACGCTAACGGAGCCTCGC 80-100
Triturus great crested newt COI 81 CGTAAACTACGGCTGACTAGTACGAA CCGATGTGTATGTAGATGCAAACA CATCCACGCTAACGGAGCCTCGC N/A
Triturus great crested newt COI 81 CGTAAACTACGGCTGACTAGTACGAA CCGATGTGTATGTAGATGCAAACA CATCCACGCTAACGGAGCCTCGC 85.5
Tropical COI 215 TTCTCCCACCTTCCTTCCTG TGCGGGTGGTTTCATGTTC N/A N/A
Urspelerpes Patch-nosed salamander CYTB N/A CGATACCGCCTCAGCCTTT CTCCGTTAGCGTGGGTGTT TTCAGTAGCCCACATCTGTCGTGA N/A
Xenopus African clawed frog 12S 83 AGGCTTAATGATTTTGCATC AGGGTATAGAAAATGTAGCC ACGTCAGGTCAAGGTGTAGCA N/A
Xenostrobus pygmy mussel COI 310 TCTATGGAYATRATYTTTCCTCG GCAGTYACAGYYATAGACCATTCCTCG TTCCTCG N/A