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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Hypophthalmichthys Silver carp N/A 168 CCACTAACATCACCACGCAA AGCCTTTTCCAGAGGCTTGG TAACCCAGCTGCCAATACAA N/A
Hypophthalmichthys Silver carp N/A 98 CCACAACTTACCCTCCTTGCC CCACAACTTACCCTCCTTGCC TCATGACATCCGCAGCATTCCTC N/A
Hypophthalmichthys Silver carp N/A 145 GGCCGGAACAGGATGAACAGTT TAATAGTTGTGGTGATGAAGTTAATTG CACGCAGGAGCATCCGTAGACCT N/A
Hypophthalmichthys Silver carp N/A 133 CRGGCCGGAACAGGATGAACAGTT TAATAGTTGTGGTGATGAAGTTAATTGGGCCGGAACAGGATGAACAGTT CACGCAGGAGCATCCGTAGACCTGGCCGGAACAGGATGAACAGTT N/A
Hypophthalmichthys bighead carp D-loop 312 TAACTTAAATAAACAGATTA TAAAAGAATGCTCGGCATGT N/A N/A
Hypophthalmichthys Silver carp N/A 133 TTCATCGGCGTAAATCTTACAT AGGGAAATAAGAGATCCGATAGA ACCCAGATGCCTACGCCCTG N/A
Hypophthalmichthys bighead carp COI 96 CGCAGGAGCATCCGTAGAC TTAATAGTTGTGGTGATGAAGTTAATTGC TTCTCCCTCCACTTAGCAG N/A
Hypophthalmichthys Bighead carp COI 120 ATCACTTCTGGGCGATGACC AGTGGCACGAGTCAGTTTCC N/A N/A
Hypophthalmichthys Bighead carp COI 141 ACTCGCGGGTAATCTTGCTC TGGGAAATGGCTGGTGGTTT N/A N/A
Hypophthalmichthys Bighead carp COI 177 AAACCACCAGCCATTTCCCA TAGGATTGGGTCTCCTCCCC N/A N/A
Hypophthalmichthys bighead carp D-loop N/A GGTGGCGCAAAATGAACTAT GCAAGGTGAAAGGAAACCAA CCCCACATGCCGAGCATTCT 88.4% - 107.7%
Hypophthalmichthys bighead carp D-loop N/A GGTGGCGCAGAATGAACTA TCACATCATTTAACCAGAT CCATGTCCGTGAGATTCCAAGCC N/A
Hypophthalmichthys Bighead Carp D-loop 100 GCGCAAAATGAACTATTACTTGCA GTA CTT TAA CCA TAT GCC AGT TAT AAT GTACAAAATGAACTATTACTTGCA 59-6FAM-ATG TCC GTG AGA TTC CAA-MGB-NFQ-39CAAAATGAACTATTACTTGCA averaged 100
Hypophthalmichthys COI 81 ACCTGATATAGCATTCCCA GAAGAGGCTAGTAGTAGAAGGAAA TAATATAAGCTTTTGACTCC 92-104
Hypophthalmichthys bighead carp COI 96 CGCAGGAGCATCCGTAGAC TTAATAGTTGTGGTGATGAAGTTAATTGC TTCTCCCTCCACTTAGCAG 102.9
Ichthyomyzon chestnut lamprey COI 194 GGCCTTCCCCCGTATAAAT ATGAGATTCCAGCGAGGTGT N/A N/A
Ichthyomyzon silver lamprey/northern brook lamprey COI 119 CTTCCACCATCATTACTTC GTCAATAGATGCTCCTGTA N/A N/A
Lampetra CYTB 126 CTTTAGCAGCAGCCATCATA GTAGTGCTAGATCAGCAATTAGAA CATTCAATTTCGTCCGC 90.43
Lampsilis wavy‐rayed lampmussel COI 122 CTCGGTGGATTTGGCCATT GAATCCGCTCAGCAACCAAT CTGTTGGGAATATACGATCC 87
Lampsilis wavy‐rayed lampmussel COI 122 CTCGGTGGATTTGGCCATT GAATCCGCTCAGCAACCAAT CTGTTGGGAATATACGATCC ???