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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Limnoperna golden mussel COI N/A TTTAGAGTTAGCACGTCCTGGTAGGTT TCCAACCAGTCCCTACTCCACCCTCTA N/A N/A
Limnoperna golden mussel COI 100 GGGACTGGTTGGACAGTTTAT ACGCACCAGCTAAATGAAGA CCCAGCAGTTGACATAGCTGCTTT N/A
Limnoperna golden mussels COI 197 AGAACCCCAGCAGTTGACATAG CCACCTAGAACTGGTAGTGAAACTAAC N/A 98
Lissotriton Smooth Newt CYTB 99 CCTACTTCTCCTACAAAGACATGCT TTTCTGGGTCTCCTAAAAGGTTTGG AAGGAGCATAAGTAAGAAACC 98
Lithobates Gopher frog ND2 82 CGGCACTACAGTCACCCTATC TCGGGATAATGGCGAGGGTAT TYCATTGACTCTTAGCCTGAGTAGGCYTA N/A
Lithobates American bullfrog COI 111 CATCCTCAACTACACAATACCAAACA GGAGTATAGTAATTC CGGCAGCTARTAAATACCAAACA CCTTTATTCGTCTGATCA GTTAATACCAAACA 98.13
Lithobates bullfrog N/A N/A TTTTCACTTCATCCTCCCGTTT GGGTTGGATGAGCCAGTTTG TTATCGCAGCAGCAAGT 101.64
Lithobates Northern green frog COI 111 CATCCTCAACTACACAATACCAAACA GGAGTATAGTAATTCCCTTTATTCGTCTGATCA GTTAATACCAAACA TCTATTTGTCTGATCAGT CTTAATACCAAACA 93.77
Lithobates Pickerel frog COI 103 CGGGACTGGCTGGACAGT TGATACCCCCGCTAA ATGTAATGAGT ATCCCCCTTTAGCCGGTAGT 104.09
Lithobates Northern leopard frog COI 100 CACACAGTACCAAACACCCCTATTT GAGTATAGTAATCCC TGCCGCTAGAAACCCCTATTT TGTTTTGATCACCGC AGT TACCCCTATTT 102.45
Lithobates North American bullfrog 12S 135 GAGAACGCCCTTTAAATCTT GTCAAGCTGACGCTCATACG ACAAACCCTCCGCCCACAAC N/A
Lithobates Mink frog COI 100 ACCATCCTCAACCACACAATACC TCCGGCAGCTAAGAC TGGAAACAATACC TATTCGTCTGGTCAG TTT TACAATACC 107.13
Lithobates Wood frog COI 130 GCCCCTCAGTAGATTTAGCTATTTTC AGRGGTGTTTGATAT TGTGTAGTTGATGATAGCTATTTTC CACCTAGCTGGTGTTTCTAGCTATTTTC 96.75
Litoria southern brown tree frog ND4 93 CGAGAATACCTTCCCTCTCAC GTGTAGGGCCAGCAGAAT AGTACCAATTCAAACCCGAGAACATGT >95%
Longnose COI 252 CAGTTGATCTAACCATTTTCTCCCTT CGTAATACCTGCGGCTAGGA N/A N/A
Lontra North American river otters CYTB N/A CCTAGCCCTAGCCCTCTCCA CCGCCGATTCATGTTAAGGT ACCTCGAAACAACGGG N/A
Lota Burbot CYTB 91 GCCGTAATACTCCTTGGCCTT CAATCGGGTTAGCGGGTGTA TGCCCTTGCCCTCTTCT-MGB 99.2
Lutra Eurasian otter CYTB 80 AAAGCCACCCTGACACGATT AGCAGGTGGATTGTTGCTAGTG TTCGCTTTCCACTTTAT 80-100
Macquaria Macquarie perch 12S 78 CCGCCTATATACCGCCGT CCTGACGTTTTGGGCTGTG N/A N/A
Macquaria Macquarie perch 12S 205 GTATAATACACCTACTATCCGCCT CTGTGCCAATTCTGCTTACTATTAGT N/A N/A