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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Danio Zebrafish Elongation factor 1 alpha CTGGAGGCCAGCTCAAACAT ATCAAGAAGAGTAGTACCGCTAGCATTAC N/A N/A
Danio Zebrafish Glyceraldehyde-3-phosphate dehydrogenase CGCTGGCATCTCCCTCAA TCAGCAACACGATGGCTGTAG N/A N/A
Danio Zebrafish Hypoxanthine guaninephosphoribosyl transferase 1 ATCAGCGAAACAGGAAAGGAG CTGCGGTGAGCTGCACTACT N/A N/A
Danio Zebrafish Porphobilinogen deaminase AAGAGCGTAATAGGCACCAGTTC GTTCTCCCAGCCCATTCTCTTC N/A N/A
Danio Zebrafish Ribosomal protein L13a TCTGGAGGACTGTAAGAGGTATGC AGACGCACAATCTTGAGAGCAG N/A N/A
Danio Zebrafish RNA polymerase subunit D CCAGATTCAGCCGCTTCAAG CAAACTGGGAATGAGGGCTT N/A N/A
Danio Zebrafish Succinate dehydrogenase complex subunit A, flavoprotein GAGTCTCCAATCAGTATCCAGTAGTAGA CACTGTGTGCGAGCGTGTTG N/A N/A
Danio Zebrafish TATA BOX binding protein CTTACCCACCAGCAGTTTAGCAG CCTTGGCACCTGTGAGTACGACTTTG N/A N/A
Danio Zebrafish Tubulin N/A TGGAGCCCACTGTCATTGATG CAGACAGTTTGCGAACCCTATCT N/A N/A
Danio Zebrafish Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide TCTGCAATGATGTGTTGGAGC TCAATGGTTGCTTTCTTGTCGTC N/A N/A
Daphnia N/A N/A TCGGAATGATCTCTCATATTATCAGTC ACCTAAGACACCAATAGCTAATATAGC TCCCAAAGGCTTCCTTCTTCCCTCTTTCG N/A
Desmognathus Northern dusky salamander COI 170 CCACGTATAAATAATATAAGCTTCTGATTA TTACC TGAAGTGAAAAGATG ATAAGCTTCTGATTA GTTAAATCTACAGA TATTTCCGGCTAAGGGCATAAGCTTCTGATTA 99.49
Desmognathus Allegheny Mountain dusky salamander COI 170 CCACGTATAAATAATATAAGCTTCTGATTA TTACC TGAAGTGAAAAGATG ATAAGCTTCTGATTA GTTAAATCTACAGA TATTTCCGGCTAAGGGCATAAGCTTCTGATTA 86.56
Dicamptodon Idaho giant salamander CYTB 78 TCTGCATCTTYCTACATATYGGAC ATC ACY CCG ACK TTT CAG GTGCATCTTYCTACATATYGGAC GCATCTTYCTACATATYGGAC N/A
Dicamptodon Idaho giant salamander CYTB N/A AAACTTTACACCAGCAAACCCATT CGGAGAATAGCGTATGCAAAAA TCAACCAGAGTGATACTT 116
Didemnum COI N/A AGCTCCTGATATAGCATTTCCTCGTTTAAA AGATATTCCTGCTAAATGTAATGAAAAAATAGCTA TAGCTCATTCAAATAGGGCAGTA 98.84
Didemnum COI N/A TGATTATTACCTTTAATAATCAGAGCTCCAGATA AGATATTCTAGCTAAATGTAGAGAAAAAATAACTA ACTGTTCATCTAGTTCTAGCTC 92.35
Dorosoma Gizzard shad ND4 101 ACTAGTCACTGTGTCGTGG TCCTCTATTCGGCTCATTCC TGCCATCCT/ZEN/TGTTCTTCTGAC N/A
Dorosoma Threadfin Shad CYTB <150 AAGTCCTCGGCCGATGTG CATGCAAACGGAGCATCCT Probe-CGTAGATACAAATGAAAAAG 104
Dreissena zebra mussel / quagga mussel 16S 139 TGGGGCAGTAAGAAGAAAAAAATAA CATCGAGGTCGCAAACCG CCGTAGGGATAACAGC 89.19