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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Ambystoma Flatwoods salamander N/A 132 GGCCCGTCAACTTTCCTCTAA TGGTCCAGGTAAATCAATTGCA TACGGTAATATGTCTGGTACTAC N/A
Ambystoma Blue-spotted salamander COI 120 AACTAAGTCAACCCGGAGCTTTAC TTCCGAATCCCCCGA TTATTGAGCTTTAC AGGTGATGACCAAAT CTACAAGAGCTTTAC 109.89
Ambystoma Spotted salamander COI 99 CGCATTCGTAATGATTTTCTTCAT AAAGCTATATCTGGT GCCCCAAT TTTCTTCAT ATGCCTGTTATAATTGGC GGATTTCTTCAT 98.42
Ambystoma Tiger salamander N/A 79 GGCAGATAGTTGGATGCACGATAG ACTACCTCTTGTCCTGGTTTTCCT CATAATATGTTGCCACGCTACT N/A
Ambystoma Tiger salamander N/A 79 GGCAGATAGTTGGATGCACGATAG ACTACCTCTTGTCCTGGTTTTCCT CALFluorRed610-CATAATATGTTGCCACGCTACT-BHQPlus 80-120%
Ambystoma Small-mouthed salamander COI 68 GCTTTTGACTATTACCCCCATCA TCCGGCACCTGCTTC AAC CCCCATCA CTTCTTCTGTTAGCC TCCTCCCCATCA 115.75
Ambystoma Tiger salamander COI 90 GAACTGTATATCCCCCACTTGCA AAACACCTGCTAAAT GAAGTGAAAAA CACTTGCA AACCTAGCCCATGCCGCACTTGCA 101.19
Ambystoma Unisexual mole sala- mander complex COI 91 CTGAGCTGGGATAGTTGGAACC ATAGATTTGGTCGTC GCCTAGCTGGAACC CGAGCAGAATTAAGCCTGGAACC 102.03
Anaxyrus Eastern American toad COI 86 GCAGGACCATCAGTTGACTTAACC GTGGTAATAAAATTA ATTGCCCCAAG GACTTAACC ACACCTGCTAGATGG AGGGACTTAACC 96.01
Anaxyrus western toad COI 111 CAAGAAAGAACCATTYGGGTACATG TCGACATTAAGATCTGTCGTAAATATGTG AATGTCTATTGGTCTTCTAGGGT 96.1
Anaxyrus western toad CYTB 92 CTACAAAGACATCTTCGGCTTCG AGTTGTCGGGGTCACCCAA ACTAATACTAGCCCTTCTAGCC 92.7
Anaxyrus Fowler’s toad COI 86 GCAGGACCATCAGTTGACTTAACC GTGGTAATAAAATTA ATTGCCCCAAGGACTTAACC ACACCTGCTAAATGA AGAGACTTAACC 102.04
Anguila N/A N/A GCAGGTATTTCATCAATTCTAGGG GAGTAGTAAAACGGCGGTTACTAA ACCGCCTGCAATTACACAGTACCA N/A
Anguilla European eel ND4 106 ATCTAGCAACGGACCCCTTA TTGGTTGGTTCTAGCCGCA ACACCACTACTAGTTTTATCTTGCT N/A
Anguilla Japanese eel 16S 153 AATCAGTAATAAGAGGGCCCAAGC TGTTGGGTTAACGGTTTGTGGTA CACATGTGTAAGTCAGAACGGACCGACC 82.69%-95.91%
Anguilla Japanese Eel 16S 153 AAACCTCGTACCTTTTGCAT CCGGTCTGAACTCAGATCACGT CACATGTGTAAGTCAGAACGGACCGACC N/A
Anguilla American eel COI 205 TAGCCCACGCCGGAGCATCT GCGGCTAGGACTGGCAGGGA N/A N/A
Anguilla American eel COI 166 CGGCGCTCCAGACATAGCGT AGATGCTCCGGCGTGGGCTA N/A N/A
Anguilla American eel COI 248 ACCAGGCGCCCTTCTTGGAG AGCCTGTACCAGCCCCAGCC N/A N/A
Anopheles Anopheles mosquito CYTB 150 TCCTAGCTATACACTATGCCGC ATTTGTCACGCTAACGGAGCT CCCACCCTTTAATTAGAATCGCTAA N/A