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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Petromyzon sea lamprey COI 225 GGCAACTGACTTGTACCMCTAATACTTGGT GGCTAAGTGTAAGGAAAAGATTGTTAGGTCGAC N/A N/A
Petromyzon Sea lamprey COI 225 GGCAACTGACTTGTACCMCTAATACTTGGT GGCTAAGTGTAAGGAAAAGATTGTTAGGTCGAC TCCCTTAGC/ZEN/CGGAAACCTAGCCCACA 93.5-111.5
Petromyzon Sea lamprey CYTB 154 GGTTTTGTTATTCTACTGGGCAT GTAGAATGGCATAGGCAAATAGA TTCCCTTTT/ZEN/AGCCCCTAATGCACT 93.5-111.5
Petromyzon Sea lamprey ND1 119 CTTACTCTCAAGTTGGCCTT CTACGTTAAAGCCAGAAACTAG TCGAACTCC/ZEN/ATTTGATTTAACTGAAGGA 93.5-111.5
Petromyzon Sea lamprey ND4 122 AACACACCTTGATCTGAAACCT AGCCTGCGATGGGAGCCT AATCGCCTG/ZEN/TTTCCTGGCCTTTT 93.5-111.5
Phocoena harbor porpoise, CYTB 43 ACACACC-CACTAATAAAAAT AGCCAAAATTTCAT-CATGAGGA (59-TCCTGGCCTTTCTATTAGTTCTTAGCA-39), N/A
Phytotriades Golden Tree Frog CYTB 80 GCGGATTCTCTGTCGACAATG TGCTCCTGCAATAAGAAATGGA CTCTCACCCGATTTTTTACAT N/A
Platichthys European flounder CYTB 104 CCGCAACAGTGATTCACCTA TGTGAAGTAGGGGTGGAAGG CCACGAAACGGGCTCAAACA 90-100
Platichthys European flounder CYTB 88 TAGGCTTTGCAGTTCTCCTT GCAGGCGTAAAGTTGTCCG CACTGGCTTCGCTCGCCCTATTTTC N/A
Plecoglossus salmonid fish CYTB 131 CCTAGTCTCCCTGGCTTTATTCTCT GTAGAATGGCGTAGGCGAAAA FAM-ACTTCACGGCAGCCAACCCCC-TAMRA 77.4-83
Plecoglossus sweetfish CYTB 131 CCTAGTCTCCCTGGCTTTATTCTCT GTAGAATGGCGTAGGCGAAAA ACTTCACGGCAGCCAACCCCC 77.4–83.0
Plecoglossus ayu CYTB 131 CCTAGTCTCCCTGGCTTTATTCTCT GTAGAATGGCGTAGGCGAAAA ACTTCACGGCAGCCAACCCCC N/A
Plethodon Eastern red-backed salamander COI 85 CCCCTTTAGCCGGAAACC GATAGATGATACTCC CGCAAGGTGACC CGCACACGCCGGAGACC 96.14
Pleuronectes European plaice CYTB 90 TAGGCTTCGCAGTCCTCCTC TTGCAGGCGTGAAGTTGTCT CTAAAAGATTTGGGGAAAATAGGGCGAGT N/A
Ponticola bighead goby CYTB 200 ACTAGGCCTATGCCTG TAGTCCTCGTCCAATATGC N/A N/A
Ponticola bighead goby CYTB 200 CTACTAGGCCTATGCCTG ATAGTCCTCGTCCAATATGC N/A N/A
Potamopyrgus New Zealand mudsnails CYTB N/A TGTTTCAAGTGTGCTGGTTTAYA CAAATGGRGCTAGTTGATTCTTT CCTCGACCAATATGTAAAT N/A
Potamopyrgus New Zealand mud snail 16S 380 CGCCTGTTTATCAAAAACAT TCAAAGATTTTGGATCATAGCT N/A N/A
Pristis largetooth sawfish COI 145 CCTCCTTCTACTAGCCTCTGCC GGA AGA GATA CCA GCT AAG TGC AACCTTCTACTAGCCTCTGCC CCTTCTACTAGCCTCTGCC N/A
Procambarus Red Swamp Crayfish COI 65 AACTAGGGGTATAGTTGAGAG CAGAAGCTAAAGGAGGATAA AGGAGTTGGAACAGGATGGACT N/A