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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Dreissena Quagga mussel COI 164 GGGGTTGAACATTATAYCCACCGTT AAACTGATGACACCCGGCACG N/A N/A
Dreissena quagga mussel COI ~600 CCTTATTATTCTGTTCGGCGTTTAG TAAACTTCAGGGTGACCAAAAAATCA N/A N/A
Dreissena Zebra mussel COI 254 GCTAAGGGCACCTGGAAGCGT N/A N/A
Dreissena Zebra mussel COI 206 GGGAAGGAGGATTCGGGGGTGG TGTGCAGAACAAAGGGACCCGGTW N/A N/A
Dreissena zebra mussel COI 136 SCCTGCGATAGATTTTTTGATTTTA GCAGAACAAAGGGACCCG CGTGCTGGATGTCAT 84.36
Dreissena zebra mussel CYTB 114 CATTTTCTTATACCTTTTATTTTATTAGTGCTTTT CGGGACAGTTTGAGTAGAAGTATCA TAGGTTTTCTTCATACTACTGGC 90.22
Dreissena zebra mussel COI <200 TGGGCACGGGTTTTAGTGTT CAAGCCCATGAGTGGTGACA CGTCCTTGGTGTGTGGGCTGGCCTTGTGGGCACGGGTTTTAGTGTTCTTATTCGTTTAGAGCTAAGGGCACCTG 99.25
Emys European pond turtle CYTB 115 CCAAATATCCTTCTGAGGTGC GCGTTATCTACTGAGAATCC N/A N/A
Engraulis Japanese Anchovy CYTB 115 GAAAAACCCACCCCCTACTCA GTGGCCAAGCATAGTCCTAAAAG CGCAGTAGTAGACCTCCCAGCACCATCC N/A
Engraulis Northern Anchovy D-loop 133 TTCACTTGGCATTTGACGGG TGCTCCTGAGATCACTTATGC AGGTTGAACATTTTCCTTGCTTGCGA 92
Entosphenus Pacific lamprey COI 126 TACCACTCATACTTAGTGCCCCTG CTGTGCCAGCCCCTGCT TTTGATTACTTCCACCCTCAC 97.2
Entosphenus Pacific Lamprey CYTB 126 CTTTAGCAGCAGCCATCATA GTAGTGCTAGATCAGCGATTAGAA TATCCAATTCCGCCCAC 90.23
Ephemera N/A N/A CTTCCTCCTGCTTTAACACTTCTT GGGCGATTCCTGCTGCTAA ACAGTTCAACCTGTTCCTGCTCCTCTTTCT N/A
Esox Northern pike COI 94 CCTTCCCCCGCATAAATAATATAA GTACCAGCACCAGCTTCAACAC CTTCTGACTTCTCCCC N/A
Esox Northern pike CR 64 AGAACCGACCAACGATTCCA CACACAATATTTGTCCGTGACCAT TCGTGCATACGGTTATT N/A
Esox Northern pike CYTB 98 GGAGTTATCCTCCTCCTCCTAACAAT TTAGTAATTACTGTTGCGCCTCAAA CTTCGTTGGCTATGTTCT N/A
Etheostoma slackwater darter CYTB N/A GTGACTTGAAAAAACCACCGTTC CAACGATCTCCGGTTTACAACAX N/A N/A
Eucyclogobius tidewater goby CYTB 119 CCTCAATTCTCGTTCTACTAGTTGT CCTAGTAGCAGACGTACTTATTCTC ACGTGCACTGACCTTCCGGCCTTTCTCC 97
Eunectes Green anaconda CYTB 134 TCGGCCTATTACCAGTAGCAA TGCTAGGTTGATGTTTGCTGT TGCTGCTTGCCTGCTCAACCCT 94.61
Eunectes Yellow anaconda CYTB 126 GGTGGAGCACTAGCCCTAACAA TGCTGCGAACAGTCAGAATGT CGTAATTCTCCTCACAGTC 95.44