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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Eurycea Northern two-lined salamander COI 72 CATAATTGGTGGGTTTGGTAACTG TTTATTCGTGGGAAG GCCATATCTGGTAACTG CTGCCACTAATAATTGGTGGTAACTG 84.96
Fasciola common liver fluke ITS-2 169 CCCCTAGTCGGCACACTTA TATGAAANTAGCATCAGACACATGA N/A N/A
Fasciola Liver Fluke ITS-2 108 GGTTGGTACTCAGTTGTCA CAAAGTGACAGTGACGGAA CCTAGTCGGCACACTTATGATTTCTG 96
Faxonius COI 124 GGAGTTGGGACAGGCTGAACAG ACT GAA CCA AGA ATA GAA GAA ACCGTTGGGACAGGCTGAACAG Not used-qPCR chemistryGTTGGGACAGGCTGAACAG not listed
Ficopomatus tubeworm COI 231 CCTTTATCCTGCTTTAGTTC AAATTCTCTAAACGAGTAGAG N/A N/A
Gadus Atlantic cod CYTB 80 TTCGCACCTAATTTACTCGGAG TCGGGCTTAACATGAGGTGG AGATAATTTCACCCCTGCTAACCCCATC N/A
Galba freshwater snail ITS-2 288 GTGAGCTCTCACGCTGCTC TAGAGCCCCTTGTTCTCCA N/A N/A
Gammarus common freshwater shrimp COI 179 CTCTAACCCTTCTACTTATAAGTAGTA GTAGAGATAAAATTAATAGCGCCG N/A N/A
Gasterosteus Three-spined stickleback CYTB 101 ACGCCACCTTAACACGTTTC AGAGCCTGTCTGGTGAAGGA CTGGTGCCACACTTGTTCAC 90-100
Globicephala long-finned pilot whale, CYTB 53 ACACATACCAATATC-TAGTCTTTCCTT CGGGCTTTAACT-TATCGTATGG (59-TCCTGGCCTTTCTATTAGTTCTTAGCA-39), N/A
Glyptemys wood turtle D-loop 134 ACAACGTTACCAGTTTCAGG CATTAACCAGAGGCCTTTTA N/A N/A
Grandidierella COI 126 GTTTTAGGTGCTTGGGCCAG AGCATGCGCTGTTACTGAGA N/A N/A
Grandidierella COI 358 CGTTTTAGGTGCTTGGGCCAG TGAGTGTGCGATGGTTGCTC N/A N/A
Grandidierella COI 126 GTTTTAGGTGCTTGGGCCAG AGCATGCGCTGTTACTGAGA N/A R2 values of the standard curves were 0.986 ∼ 0.995
Grandidierella COI 358 CGTTTTAGGTGCTTGGGCCAG TGAGTGTGCGATGGTTGCTC N/A R2 values of the standard curves were 0.986 ∼ 0.995
Gymnocephalus Ruffe COI 111 TACCCTCCCCTATCAGGAAACTT TAATTGCGCCCAAGATTGAGGAGAT N/A N/A
Gyrodactylus ITS GGTGGTGGCGCACCTATTC ACGATCGTCACTCGGAATCGAT CAAGCAGAACTGGTTAAT 100
Gyrodactylus ITS 60 CGATCGTCACTCGGAATCG GGTGGCGCACCTATTCTACA TCTTATTAACCAGTTCTGC (Two effieciences): Multiplex-95.7; Singleplex-93.1
Halyomorpha brown marmorated stink bug ITS-1 96 CGAGGCCGCCGATGA CCCACGAGCCGAGTGATC CAGGCAATGAAGCACA 97.6
Hemichromis African jewelFish COI 64 CCCTCTAGCAGGCAACCTC GTGGAGGGAGAAGATGGCTA CCACGCCGGACCTTCCGTAGAC N/A