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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Anopheles Anopheles mosquito CYTB 150 AGCTATACACTATGCCGCAGAT AAGCTCCGTTAGCGTGACAAA CGGCATAGTGTATAGCTAGGAATAAT N/A
Aphanomyces Saprolegniaceae (family) ITS-1 59 AAGGCTTGTGCTGGGATGTT CTTCTTGCGAAACCTTCTGCTA TTCGGGACGACCC N/A
Ascaphus Rocky mountain tailed frog CYTB 90 ACGTCAACTATGGCTGGCTAATC GTCCTCGGCCAATGTGAAGA CATGCAAATGGAGCATC 118
Ascaphus Rocky Mountain tailed frog CYTB 85 CGTCAACTATGGCTGGCTAA TCG GCC AAT GTG AAG ATA AACAACTATGGCTGGCTAA CAACTATGGCTGGCTAA N/A
Ascaphus Rocky Mountain tailed frog CYTB 176 ACTTTATTACGGCTCTTACTTG GTACGTTTCCGATGTAAGGGA ATACGTATTACCATGAGGACAAATATC N/A
Ascaphus Coastal tailed frog CYTB 202 GAACATTGGCATTATCCTACTT AGGCGAAAAATCGTGTTAAC CGCTTTTGTAGGGTATGTGTTACCG N/A
Asellus waterlouse COI 174 GGCAATGACCAGATTTACAATGTAA ATTTATACGAGGAAATGCTATATCTGG N/A N/A
Astacus noble crayfish COI 65 GATTAGAGGAATAGTAGAGAG CTGATGCTAAAGGGGGATAA Fam-AGGAGTAGGGACAGGATGAACT-BHQ-1 97.4
Astacus narrow-clawed crayfish COI 65 AACTAGGGGTATAGTAGAGAG CTGATGCTAAAGGGGGATAA Fam-AGGAGTAGGGACCGGATGAACT-BHQ-1 88.78
Astacus narrow-clawed crayfish COI 65 AACTAGAGGTATAGTAGAGGG CTGATGCTAGGGGAGGATAA Fam-GGGTGTAGGAACTGGATGAACC-BHQ-1 88.78
Atractosteus Alligator Gar COI 159 TAAAACCACCCGCAGCTTCC CCTGCAGGGTCAAAGAAGGT N/A N/A
Atractosteus COI 325 CCAGTTATAATTGGCGGGTTC TGCGGGTGGTTTTATGTTTAGG N/A N/A
Austropeplea Snail ITS-2 118 GCCAAATTTTCCTCCTCGT AAGCGAGCGTCAGCGTAA CTAACGGGCCCGCTCGTAACA 90
Austropotamobius white-clawed crayfish 16S 83 AGTTACTTTAGGGATAACAGCGT CTTTTAATTCAACATCGAGGTCG N/A 93.8
Babka Ponto-Caspian goby CYTB 125 CCTCTAACATTTCTGCC GACGAGAAGGCTGTGGC N/A N/A
Babka Ponto-Caspian goby CYTB 125 CCCTCTAACATTTCTGCC GACGAGAAGGCTGTGGC N/A N/A
Boa Boa constrictor CYTB 83 CATGGTGAAACTTCGGATCAATAC TGTGTAATGTACGGCCAAGAAGA ATGCTCCATAATTCAAGTAC 90.68
Bugula Bryozoan COI 185 GGTACATTATACTTTTTATTTGGAC CCCCCAATTATAACTGGTATG CCAGGCAGCTTATTAGGTAACGACCA 104
Bythotrephes spiny water flea COI 227 GCAGGAACTGGCTGAACA AATAATAAAAGGAGGGCTGTAATACC N/A N/A
Bythrotrephes Spiny waterflea COI 248 GCTGAGTTGGGACAGGCAGG CCACTCTCTACGGCCCCTCC N/A N/A