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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Cyprinella COI 143 GGGCAGTTAACTTCATTACTAC AGTAATTCCGGCAGCTAAC AGTATCAGACACCTTTG 92-104
Cyprinus common carp CYTB 149 CTAGCACTATTCTCCCCTAACTTAC ACACCTCCGAGTTTGTTTGGA (6FAM) CCCTCTAGTTACACCACC (MGBNFQ) 92
Cyprinus common carp D-loop 146 GAGTGCAGGCTCAAATGTTAAA GTAAGGATAAGTTGAACTAGAGACAG N/A 81-97
Cyprinus Common Carp N/A 146 GAGTGCAGGCTCAAATGTTAAA GTAAGGATAAGTTGAACTAGAGACAG N/A 97.2
Cyprinus common carp 12S 73 CAAACTGGGATTAGATACCCCACTAT CTGGCGGACATCTAATTGTAGC CAGCCGTAAACTC 0.86–0.99
Cyprinus Common carp D-loop N/A TCCACCCTCGGATAAT ACTATGTAAGGATAAGTTGAACTA GAGATGCCAGA 79
Cyprinus Common carp D-loop N/A TCCACCCTCGGATAAT ACTATGTAAGGATAAGTTGAACTA GAAATGCCAGA 83
Cyprinus Common carp ITS1 84 TTCAAAGACCCCCCGTAAC GCCATGCCGCACACA TCACGACCCCCCTTATTTTTTCCAAAACC 96.1 ± 12.5%
Cyprinus common carp CYTB 78 GGTGGGTTCTCAGTAGACAATGC GGCGGCAATAACAAATGGTAGT CACTAACACGATTCTTCGCATTCCACTTCC 88.5-102.5
Cyprinus common carp CYTB N/A GGTGGGTTCTCAGTAGACAATGC GGCGGCAATAACAAATGGTAGT CACTAACACGATTCTTCGCATTCCACTTCC 0.984 to 0.990
Cyprinus common carp CYTB 78 GGTGGGTTCTCAGTAGACAATGC GGCGGCAATAACAAATGGTAGT CACTAACACGATTCTTCGCATTCCACTTCC N/A
Cyprinus common carp/goldFish COI 171 AGCCCACGCAGGAGCATCAG ACGGCGGTTACAAGCACGGA N/A N/A
Dactylogyrus ITS-1 185 GCCATCCTTGAGGGAATATGCCCA GAGTTTACGTTGACCGCCCGACAT N/A 75.12
Dactylogyrus ITS-1 184 ATCATCCTTGTGGGAATCTGCCCG AAGTGTACGTTGACCGCCAGCAG N/A 119.55
Dactylogyrus ITS-1 210 TCAGAATCTGAACCCTATCCAATAC TGCCGCACGACACGTTA N/A 104.6
Dactylogyrus ITS-1 120 TCGTCGTGACGACCTTGG CACATACTGCAGTGACCCT N/A 97.3
Dactylogyrus ITS-1 210 GTTGCGGAACTGAACCCTAGCCA AGACTGCACGACACGTTACCAA N/A 98.99
Danio zebrafish COI 95 ATCATAAAGACATTGGCACCCTG GCTAAGTTCAGCTCGGATTAAG N/A N/A
Danio Zebrafish Beta 2 microglobulin AGGATTGTCTGCTTGGCTCTCT GGAGTGGAGACTTTCCCCTGTAC N/A N/A
Danio Zebrafish Beta actin N/A CGAGCTGTCTTCCCATCCA TCACCAACGTAGCTGTCTTTCTG N/A N/A