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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Pseudorasbora topmouth gudgeon COI 154 ATTCTCATTGGGGGCTTCGG TTCCTGCTCCAGCCTCTACT N/A N/A
Ptychobranchus kidneyshell COI 129 TTGGTAATTGACTTGTTCCTCTTATGA CACTCTCAACTAAAGAAGAACTCAACAATA CTCGATTAAACAATTTGAGC 100
Ptychobranchus kidneyshell COI 129 TTGGTAATTGACTTGTTCCTCTTATGA CACTCTCAACTAAAGAAGAACTCAACAATA CTCGATTAAACAATTTGAGC ???
Ptychocheilus Sacramento Pikeminnow COI <150 TCTTCGTATGGGCCGTACTTG GCCAGGACTGGTAGTGATAACAGA Probe-AACAGCCGTTCTTC 103
Python Burmese python CYTB <150 ACCATACAAGTATTAACCGG GTATGGAACATCGCGGG N/A N/A
Python Burmese Python ND4 146 CACCCTAACAACTTCAATACCTCTACTAAT GAG GTT TGT TCA GTGGTT ATT TGT TTTCCTAACAACTTCAATACCTCTACTAAT CCA ACA CTA TTA TTCCTA GCA ACCCTAACAACTTCAATACCTCTACTAAT 1.0805
Python Burmese python CYTB 146 CACCCTAACAACTTCAATACCTCTACTAAT GAGGTTTGTTCAGTGGTTATTTGTTTT CCAACACTATTATTCCTAGCAAC 108.05% and 92.72%
Python North African python CYTB 67 AATCACCAACCTACTCACTGCTGTAC GCCTCCCCATAATCAGGTTGT CTACCTAGGAACAACTCT 92.85
Quadrula mapleleaf COI 99 AGCGGATTCCTTTGTTTGTATGA CCGTAAGTAGCATCGTGATAGCA TTGCGGCGTTACCTGT 97
Quadrula mapleleaf COI 99 AGCGGATTCCTTTGTTTGTATGA CCGTAAGTAGCATCGTGATAGCA TTGCGGCGTTACCTGT ???
Rana Northern red-legged frog CYTB 85 CGGCTGACTCCTCCGTAATTTA ATAGAGGCCACGTCCGATGT ATGCTAACGGCGCATC 95–109%
Rana American bullfrog CYTB 79 TGCCAACGGAGCATCATTC ATAAAGGTAGGAGCCGTAGT N/A N/A
Rana American bullfrog 16S 79 GCAGAGATAACCTCTCGT GTCCCATAGGACTGTTCT TGCCCTCCCGAAACTAAGTGAGC 76%-100%
Rana Chiricahua leopard frog N/A 84 GGTACCGCTCATATCATGACTACTTG TCCAGTTGGACTCACTTAGGAATG TAGGACCTTCGCTTGTTAT N/A
Rana Chiricahua leopard frog N/A 84 GGTACCGCTCATATCATGACTACTTG TCCAGTTGGACTCACTTAGGAATG 6FAM- TAGGACCTTCGCTTGTTAT-MGB 80-120%
Rana California red-legged frog CYTB 98 CATCTGCCGCGATGTTAATAAC CTCGCCCGATGTGGAAGTAG CATCCTTCTTTTTTATCTGCA 95–109%
Rana wood frog CYTB 115 TCCTTCATCAAACAGGATCATCTA CCTAGTATAATGGTGAAGCCGAAT N/A N/A
Rhinella Cane Toad spanning part of the mitochondrial tRNA-Gly and NADH dehydroge- nase subunit 3 (ND3) genes 80 ACCCCAGGAGAAAATAATGTCTCT ACCAGAAGCTAACAGTGGCTAAAAT CAATTGCTAGGGTAATAAA 100
Rhinichthys Loach Minnow CYTB 163 CTTACCCAGTTCCTATTTTGGACACT ATTCTCTATCCATCCTGCGAGC TGGCGGATATACTCATCCT 100.3
Salmo Atlantic Salmon COI 214 AGCAGAACTCAGCCAGCCT AAAGGAGGGAGGGAGAAGTCAA CCTTCTGGGAGATGACC 93.4