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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Salmo Atlantic salmon CYTB 95 CGGAGCATCTTTCTTCTTTATCTGT ACTCCGATATTTCAGGTTTCTTTATATAGA CCAACAACTCTTTAACCATC 90.6
Salmo Atlantic salmon COI 74 CGCCCTAAGTCTCTTGATTCGA CGT TAT AAA TTT GGT CAT CTC CCA GACCTAAGTCTCTTGATTCGA (5’-AGA ACT CAG CCA GCC TG-3’)CCTAAGTCTCTTGATTCGA 100.018
Salmo Atlantic Salmon D-loop 230 ACCCCTAAACCAGGAAGTCT TGCTTTAGTTAAGCTACGCCAACT N/A 101
Salmo Brown trout COI 61 TTTTGTTTGGGCCGTGTTAGT TGCTAAAACAGGGAGGGAGAGT ACCGCCGTCCTCT 93
Salmo Brown trout CYTB N/A CGCCCGAGGACTCTACTATGGT GGAAGAACGTAGCCCACGAA CGGAGTCG TACTGCTAC 99.1
Salvelinus CYTB 145 CCGCCACAGTACTTCACCTTCTA AGGCCAAGCAATATAGCTACGAAA CCGACAAAATCTCATTCC 99.9
Salvelinus arctic char CYTB 145 CCGCCACAGTACTTCACCTTCTA AGGCCAAGCAATATAGCTACGAAA FAM-CCGACAAAATCTCATTCC-MGB-NFQ 99.9
Salvelinus Arctic char CYTB 145 CCGCCACAGTACTTCACCTTCTA AGGCCAAGCAATATAGCTACGAAA CCGACAAAATCTCATTCC N/A
Salvelinus bull trout CYTB 172 TTCCTTTTGCCTAGGGTAGCG CGATACTCAACACGCTTCACAATT FAM-CCACGGCCACACGG-MGBNFQ N/A
Salvelinus bull trout CYTB approx. 150 AGTACTTCACCTTCTGTTTCTGCATG CAATATAGCTACGAAACCGAGGAGG CCGACAAAATCTCA N/A
Salvelinus brook trout CYTB ~150 CCACAGTGCTTCACCTTCTATTTCTA GCCAAGTAATATAGCTACAAAACCTAATAGATC ACTCCGACGCTGACAA 90-110%
Salvelinus brook trout CYTB N/A CCACAGTGCTTCACCTTCTATTTCTA GCCAAGTAATATAGCTACAAAACCTAATAGATC ACTCCGACGCTGACAA 89.1
Salvelinus brook trout CYTB 90 CCATGAGGGCAAATATCCTTCTGA TCATTGTACAAGGGCACCTCCTA CTCCTCTCTGCTGTACCC 90.4%-101.1%
Salvelinus brook trout CYTB approx. 150 CCACAGTGCTTCACCTTCTATTTCTA GCCAAGTAATATAGCTACAAAACCTAATAGATC ACTCCGACGCTGACAA 85-106 (mean 94)
Salvelinus Brook Trout COI 103 CGGTACGGGGTGAACAGTTT GGAAATGCCAGCTAAATGTAGGG CTCGCCCACGCAGGAGCTTC 100
Salvelinus Brook trout COI 103 CGGTACGGGGTGAACAGTTT GGAAATGCCAGCTAAATGTAGGG CTCGCCCACGCAGGAGCTTC N/A
Salvelinus brook trout CYTB 139 CCACAGTGCTTCACCTTCTATTTCTA GCCAAGTAATATAGCTACAAAACCTAATAGATC ACTCCGACGCTGACAA 99.6
Salvelinus brook trout #1 CYTB approx. 150 CCATGAGGGCAAATATCCTTCTGA TCATTGTACAAGGGCACCTCCTA CTCCTCTCTGCTGTACCC N/A
Salvelinus brook trout #2 CYTB approx. 150 CCACAGTGCTTCACCTTCTATTTCTA GCCAAGTAATATAGCTACAAAACCTAATAGATC ACTCCGACGCTGACAA 92.24
Salvelinus Whitespotted char CYTB 126 CCCAGCAGGGATCAACTCAG GGGTTGGCTGGCGTGA CCTAACAGCCCTAGCTC N/A