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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Silurus Wels catFish COI 150 TCGGAGGGTTTGGAAACTGGCTTGTG CTGTTCCTGCGCCCGCTTCG N/A N/A
Silurus Wels catfish COI 117 GACTTCTCCCTCCTTCATTCCTG AAGCACCTGCGTGGGCG CGGAGTCGAAGCGGGC 92-104
Sinanodonta COI 258 GGGTCAGCCMGGRAGGCTTTTA TGTTCACCCTGTACCAACRCCC N/A N/A
Siniperca COI 149 CTTAGCAGGAATTTCTTCCATCCTT CGGGTAGGGAGAGCAGAAGAAGT ACCCCTCTGTTCGTGTGG 92-104
Sistrurus eastern massasauga rattlesnake COI 137 CACTACTTCTCCTACTCTCCTC GGCCAAATGAAGGGAGAAA AACAGTTCATCCTGTCCCTGCGCC N/A
Sphaerothecum 18S N/A ACTTTGCGAATCGTATGACATTTTGTC CCACTACCTTACCATCGAAAGTTGA ACGATGATTCATTCAAATTTC N/A
Spirinchus Longfin Smelt CYTB <150 CTCTGCCGGGACGTCAAT CCCGTTAGCGTGCATATTCC Probe-ACGGCTGACTAATC 94.2
Stygobromus Hay’s spring amphipod COI 126 GCATCTGTCGACTTAGCTATT CGGCACTTGGTCTATAGTTATT TCACTTCATTTAGCAGGAGCCTCCTC N/A
Stygobromus potomac stygobromid COI 154 CTGAACAGTATATCCACCACT CATTCCAGGTCTCCGTATATTA TGCAGTAGCCCATAGTGGAGCATCT N/A
Sus Wild Pig D-loop 101 CAAGCATTCCATTCGTATG CGCATATTTGTATGTTTGTG AAACCAAAACGCCAAGTACTTAATTAC 90-110
Sus Wild Pig D-loop 101 CAAGCATTCCATTCGTATG CGCATATTTGTATGTTTGTG AAACCAAAACGCCAAGTACTTAATTAC 95.1
Tetracapsuloides Tetracapsuloides bryosalmonae 18S 170 CGAACGAGACTTCTTCCTT CTTCCTACGCTTTTAAATAGCG CCCTTCAATTAGTTGATCTAAACCCCAATT 97.76
Thymallus arctic grayling COI 116 TGTGGGCTGTTCTGATTACCG TGCTGGGTCAAAGAAAGTGGTATTA FAM-CTTGCAGCAGGTATC-MGB-NFQ 1.001
Thymallus Arctic grayling COI 116 TGTGGGCTGTTCTGATTACCG TGCTGGGTCAAAGAAAGTGGTATTA CTTGCAGCAGGTATC 100.1
Trachurus Japanese jack mackerel CYTB 127 CAGATATCGCAACCGCCTTT CCGATGTGAAGGTAAATGCAAA TATGCACGCCAACGGCGCCT 94.410 ± 3.821
Trachurus Japanese jack mackerel CYTB 127 CAGATATCGCAACCGCCTTT CCGATGTGAAGGTAAATGCAAA TATGCACGCCAACGGCGCCT N/A
Trachurus Japanese jack mackerel CYTB 719 AATCCTCACAGGTCTTTTCCTAGCTA ATTGATCGGAGAATGGCGTATG TACCATTCGTCATTGCAGCCTTCTTTGTTC N/A
Trachurus Japanese jack mackerel CYTB 127 CAGATATCGCAACCGCCTTT CCG ATG TGA AGG TAA ATG CAA AATATCGCAACCGCCTTT TAT GCA CGC CAA CGG CGC CTATATCGCAACCGCCTTT range: 79.10 to 98.03
Trichechus Antillean manatee CYTB 69 CGCTAACCGCATTCTCTTCAG GGT AGC GAA TGA TYCAAC CAT AGT TTAACCGCATTCTCTTCAG (5’-CCC ACA TTT GCC GAGAC-3’)TAACCGCATTCTCTTCAG 90-110%
Trichechus Florida manatee CYTB 69 CGCTAACCGCATTCTCTTCAG GGT AGC GAA TGA TYCAAC CAT AGT TTAACCGCATTCTCTTCAG (5’-CCC ACA TTT GCC GAGAC-3’)TAACCGCATTCTCTTCAG 90-110%