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Genus Common Name Gene Region Fragment Length Forward Primer Reverse Primer Probe PCR Efficiency
Oncorhynchus Rainbow (steelhead) trout COI 121 ACCATTATTAACATAAAACCTCCAG GTAATGCCTGCTGCCAGGA CGTTTGAGCCGTGCTA N/A
Oncorhynchus rainbow trout ND1 102 AGTCTCTCCCTGTATATCGTC GATTTAGTTCATGAAGTTGCGTGAGTA CCAACAACTCTTTAACCATC 95.9
Oncorhynchus Sockeye salmon COI 152 GGAAACCTTGCCCACGCG AAAAGTGGGGTCTGGTACTGAG CTCTGTTGACTTAACCATC N/A
Oncorhynchus sockeye salmon COIII 70 TCTGCCCTTCTCCTTACGATTTT GTTCGACCTAGAAATCGCCCTT CCATCCTGTTCCTCCT 86.08
Oncorhynchus Chinook salmon COI 90 CTGGCACMGGGTGAACAGTCTACC AATGAAGGGAGAAGATCGTYAGATCA CTCCTGCGTGGGCTAG N/A
Oncorhynchus Chinook Salmon COI 90 CTGGCACMGGGTGAACAGTCTACC AAT GAA GGG AGA AGA TCG TYA GAT CAGCACMGGGTGAACAGTCTACC CTC CTG CGT GGG CTA GGCACMGGGTGAACAGTCTACC 94.83
Oncorhynchus Chinook salmon COI 183 GATAGTAGGCACCGCCCTTAGT CCGATCATTAGGGGAATTAATCAGT TCATAATCGGCATAACTAT 98
Oncorlynchus Chinook Salmon CYTB <150 CCTAAAAATCGCTAATGACGCACTA GGAGTGAGCCAAAGTTTCATCAG Probe-AGCACCCTCTAACATTTCAG 93.1
Onychodactylus Japanese clawed salamander 12S 123 TACTTGAAACCACGACCGCT CGCCAAGTCCTTTGAGTTTT TCCGCCAGATTACTACGAGC 78.45 to 95.65%.
Ophidomyces Snake fungal disease COI N/A TGTTTCTGTCTCGCTCGAAGAC AGGTCAAACCGGAAAGAATGG CGATCGGGCGCCCGTCGTC N/A
Opisthorchis N/A COI 138 GGTGGTTTGGGCTCATCATA GAAC CCAA CTAT CCAC CACA TAAGGGCTCATCATA OV-COI-P (5-FAM- TAGCT CGGTT ACTAT GATTA T -NFQ-MGB-3),GGGCTCATCATA N/A
Opisthorchis Southeast liver fluke COI ≈103 GCTGGATTTGGGCACCG AGTACCCGCAAGCATATACAACC TAGCTCGGTTACTATGATTAT N/A
Opsariichthys Amur Three-Lips D-loop N/A CATTTCCTTGCCAGGCTTAATAATA GCAAAAGGGGGCATATATATAAGAGA 5'-FAM-CATATGTTTATCTCATGTGCATAAC-TAMRA-3' N/A
Opsariichthys Three-lips (Fish) D-loop 129 CATTTCCTTGCCAGGCTTAATAATA GCAAAAGGGGGCATATATATAAGAGA CATATGTTTATCTCATGTGCATAAC 67.2%–82.4%
Orconectes spinycheek crayFish COI 78 CCTCCTCTCGCTTCTGCAAT AACCCCTGCTAAATGCAACG CTCATGCAGGGGCATCAGTGG N/A
Orconectes rusty crayfish COI 128 CAGGGGCGTCAGTAGATTTAGGTAT CATTCGATCTATAGTCATTCCCGTAG N/A 0.95-1.05
Orconectes rusty crayFish COI 128 CAGGGGCGTCAGTAGATTTAGGTAT CATTCGATCTATAGTCATTCCCGTAG N/A 95-100%
Oreochromis Mozambique tilapia 16S 189 CTTCAGACGCCAGAACAG GCTTGGAGTTGTAACTCTGG N/A R^2 values: 0.98187 to 0.99999; efficiencies ranged from 0.94 to 1.21
Oreochromis Nile tilapia COI 154 GGCCGGGGTGTCATCTATTT GGCAAGAACGGGTAGGGATAG N/A N/A
Ornithorhynchus platypus CR 57 CAGCAATACCCTAGACAAGG CGCTTCAATGGCTGCGC CGAACCCCATGAGTAGAAAAT N/A